LOCUS       40924_22899       11805 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB414 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S.
  TITLE     Improved Gateway Binary Vectors: High-Performance Vectors for 
            Creation of Fusion Constructs in Transgenic Analysis of Plants
  JOURNAL   Biosci. Biotechnol. Biochem. (2007) In press
  PUBMED    17690442
REFERENCE   2  (bases 1 to 11805)
  AUTHORS   Nakagawa T, Ishiguro S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, 
            Center for Integrated Research in Science; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan 
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595, 
            Fax:81-852-32-6109)
REFERENCE   3  (bases 1 to 11805)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11805)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem. (2007) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, Center for 
            Integrated Research in Science"; volume: " 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan 
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595, Fax"; 
            pages: "81-852-32-6109"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2771..2895)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2924..3013
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      3047..3299
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3333..3349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3357..3373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3381..3411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3426..3447)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3481..3733)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4126..4914)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4938..5121)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5327..5351)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5872..6660
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6909..7497
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7683..7823)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8167..8361)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8430..9500)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9932..10558)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgggtt aattaacatc ttttacccat acgatgttcc
     2941 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
     3001 tccagattac gctgctcagt gcagcgctta gagctcgaat ttccccgatc gttcaaacat
     3061 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     3121 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     3181 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     3241 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     3301 ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3421 gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt ctgaattccc
     3481 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
     3541 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     3601 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     3661 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     3721 tgtttgaacg atcggggatc atccgggtct gtggcgggaa ctccacgaaa atatccgaac
     3781 gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga
     3841 tgtggacgcc gggcccgatc atattgtcgc tcaggatcgt ggcgttgtgc ttgtcggccg
     3901 ttgctgtcgt aatgatatcg gcaccttcga ccgcctgttc cgcagagatc ccgtgggcga
     3961 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga
     4021 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt
     4081 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag
     4141 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     4201 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     4261 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     4321 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat catcgccgtc
     4381 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     4441 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     4501 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     4561 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     4621 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     4681 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     4741 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     4801 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     4861 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
     4921 aaacgatcca gatccggtgc agattatttg gattgagagt gaatatgaga ctctaattgg
     4981 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     5041 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     5101 ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt
     5161 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     5221 ttctccgctc atgatgaatt cagaaataaa ttcagcctaa ttcggcgtta attcagtaca
     5281 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     5341 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     5401 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     5461 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     5521 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     5581 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     5641 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     5701 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     5761 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     5821 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     5881 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     5941 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     6001 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     6061 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     6121 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     6181 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     6241 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     6301 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     6361 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     6421 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     6481 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     6541 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     6601 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     6661 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     6721 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     6781 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     6841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7021 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7561 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7621 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7681 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     7741 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     7801 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     7861 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7921 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     7981 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
     8041 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     8101 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
     8161 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     8221 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     8281 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     8341 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
     8401 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     8461 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     8521 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     8581 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
     8641 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
     8701 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
     8761 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
     8821 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
     8881 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
     8941 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
     9001 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
     9061 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
     9121 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
     9181 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
     9241 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
     9301 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
     9361 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
     9421 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
     9481 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
     9541 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
     9601 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
     9661 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
     9721 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
     9781 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
     9841 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
     9901 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
     9961 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    10021 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    10081 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    10141 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    10201 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    10261 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    10321 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    10381 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    10441 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    10501 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    10561 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    10621 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    10681 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    10741 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    10801 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    10861 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    10921 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    10981 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    11041 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    11101 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    11161 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    11221 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    11281 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    11341 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    11401 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    11461 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    11521 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    11581 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    11641 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    11701 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    11761 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aacct
//
